15 Mhando dzeHuman Papillomavirus E6/E7 Gene mRNA
Product name
HWTS-CC005A-15 Mhando dzeHuman Papillomavirus E6/E7 Gene mRNA Detection Kit (Fluorescence PCR)
Epidemiology
Gomarara remuchibereko nderimwe remhando dzegomarara remunhukadzi pasi rose, uye kuitika kwaro kune hukama hwepapillomaviruses (HPV), asi chikamu chidiki cheutachiona hweHPV chinogona kuita gomarara.High-risk HPV inobata cervical epithelial cells uye inogadzira maoncoprotein maviri, E6 uye E7.Iyi puroteni inogona kukanganisa akasiyana-siyana mapuroteni emasero (akadai sebundu suppressor mapuroteni pRB uye p53), kurebesa kutenderera kwesero, kukanganisa DNA synthesis uye genome kugadzikana, uye kukanganisa antiviral uye antitumor immune mhinduro.
Channel
| Channel | Chikamu | Genotype yakaedzwa |
| FAM | HPV Reaction Buffer 1 | HPV16,31,33,35,51,52,58 |
| VIC/HEX | Munhu β-actin gene | |
| FAM | HPV Reaction Buffer 2 | HPV 18, 39, 45, 53, 56, 59, 66, 68 |
| VIC/HEX | Munhu INS gene |
Technical Parameters
| Storage | Mvura: ≤-18℃ |
| Sherufu-hupenyu | 9 mwedzi |
| Type Type | cervical exfoliated cell |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Makopi/mL |
| Zvishandiso Zvishandiso | Applied Biosystems 7500 Chaiyo-Nguva PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Chaiyo-Nguva PCR Systems SLAN-96P Chaiyo-Nguva PCR Systems LightCycler®480 Chaiyo-Nguva PCR system LineGene 9600 Plus Chaiyo-Nguva PCR Detection System MA-6000 Chaiyo-Nguva Quantitative Thermal Cycler BioRad CFX96 Chaiyo-Nguva PCR System BioRad CFX Opus 96 Chaiyo-Nguva PCR System |
Kuyerera Kwebasa
Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kubvisa kunofanira kuitwa zvakanyatsoenderana nemirairo yekushandisa. .Iyo yakakurudzirwa elution vhoriyamu i50μL.Kana sampuli isina kunyatsogayiwa, idzorere kudanho rechina rekugaya zvakare.Uye wozoyedza zvinoenderana nemirairo yekushandisa.
Inokurudzirwa kuburitsa reagent: RNAprep Yakachena Mhuka Tissue Yese RNA Extraction Kit (DP431).Kudhirowa kunofanirwa kuitwa zvinoenderana nemirairo yekushandisa zvakasimba (Mudanho rechishanu, kaviri kuwanda kweDNaseI kushanda mhinduro, kureva, tora 20μL yeRNase-Yemahara DNaseI (1500U) stock solution muRNase-Mahara centrifuge chubhu, wedzera 60μL yeRDD buffer, uye sanganisa zvinyoro nyoro).Iyo yakakurudzirwa elution vhoriyamu i60μL.Kana sampuli isina kunyatsogayiwa, idzorere kune nhanho yechishanu yekugaya zvakare.Uye wozoyedza zvinoenderana nemirairo yekushandisa.
