Herpes Simplex Virus Type 2 Nucleic Acid
Product Name
HWTS-UR007A-Herpes Simplex Virus Type 2 Nucleic Acid Detection Kit (Fluorescence PCR)
Chinangwa Kushandiswa
Iyi kit inoshandiswa pakuonekwa kwemhando yeherpes simplex virus type 2 nucleic acid mumurume urethral swab uye echikadzi cervical swab samples.
Epidemiology
Herpes Simplex Virus Type 2 (HSV2) hutachiona hwedenderedzwa hwakagadzirwa ne tegument, capsid, musimboti, uye envelope, uye ine kaviri-yakasungwa mutsara DNA.Utachiona hweHerpes hunogona kupinda mumuviri kuburikidza nekubata zvakananga kana kusangana pabonde neganda uye mucous membranes, uye yakakamurwa kuva yekutanga uye inodzokororwa.Utachiona hwehutachiona hunonyanya kukonzerwa neHSV2, varwere vechirume vanoratidzwa semaronda epenile, uye varwere vechikadzi vanoratidzwa semuromo wekervical, vulvar, uye maronda emukadzi.Zvirwere zvekutanga zvegenital herpes virus kazhinji hutachiona hwehutachiona, kunze kweashoma emunharaunda herpes ane mucous membrane kana ganda, mazhinji ayo asina pachena zviratidzo zvekiriniki.Genital herpes utachiona hune hunhu hwehupenyu hwese hutakura hutachiona uye nyore kudzoka, uye vese varwere nevatakuri ndivo tsime rehutachiona hwechirwere.MuChina, serological positive rate yeHSV2 inenge 10.80% kusvika 23.56%.Nhanho yehutachiona hweHSV2 inogona kukamurwa kuita hutachiona hwekutanga uye hutachiwana huchidzokororwa, uye vangangoita 60% yevarwere vane HSV2 vanodzoka zvakare.
Epidemiology
FAM: Herpes Simplex Virus Type 2 (HSV2)·
VIC (HEX): Kudzora Kwemukati
PCR Amplification Conditions Setting
Danho | Cycles | Tembiricha | Nguva | UnganidzaFluorescentSiignalskana Kwete |
1 | 1 Cycle | 50 ℃ | 5 mins | No |
2 | 1 Cycle | 95℃ | 10mins | No |
3 | 40 Cycles | 95℃ | 15secs | No |
4 | 58℃ | 31secs | Ehe |
Technical Parameters
Storage | |
Liquid | ≤-18℃ Murima |
Sherufu-hupenyu | 12 mwedzi |
Type Type | Mukadzi cervical swab, Male urethral swab |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 50Makopi/maitiro |
Zvakananga | Iko hakuna kuyambuka-reactivity nemamwe hutachiona hweSTD, seTreponema pallidum, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium uye nezvimwe. |
Zvishandiso Zvishandiso | Inogona kuenderana neyakajairwa fluorescent PCR zviridzwa pamusika. Applied Biosystems 7500 Chaiyo-Nguva PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Chaiyo-Nguva PCR Systems SLAN-96P Chaiyo-Nguva PCR Systems LightCycler®480 Chaiyo-Nguva PCR system LineGene 9600 Plus Chaiyo-Nguva PCR Detection System MA-6000 Chaiyo-Nguva Quantitative Thermal Cycler BioRad CFX96 Chaiyo-Nguva PCR System BioRad CFX Opus 96 Chaiyo-Nguva PCR System. |