Munhu BRAF Gene V600E Mutation
Product name
HWTS-TM007-Munhu BRAF Gene V600E Mutation Detection Kit(Fluorescence PCR)
Chitupa
CE
Epidemiology
Anopfuura makumi matatu marudzi eBRAF shanduko akawanikwa, ayo angangoita makumi mapfumbamwe muzana ari mu exon 15, apo V600E shanduko inoonekwa seyakajairika shanduko, ndiko kuti, thymine (T) pachinzvimbo 1799 mu exon 15 inoshandurwa kuita. adenine (A), zvichikonzera kutsiviwa kwevaline (V) pachinzvimbo 600 neglutamic acid (E) muprotein product.Kuchinja kweBRAF kunowanzo kuwanikwa mumamota akashata senge melanoma, colorectal cancer, cancer cancer, uye cancer yemapapu.Kunzwisisa shanduko yeBRAF gene kwave kudiwa kwekuongorora EGFR-TKIs uye BRAF gene mutation-inotangwa mishonga mukiriniki yakanangana nemishonga kurapwa kune varwere vanogona kubatsirwa.
Channel
| FAM | V600E shanduko, kutonga kwemukati |
Technical Parameters
| Storage | ≤-18℃ |
| Sherufu-hupenyu | 9 mwedzi |
| Type Type | parafini-embedded pathological tissue samples |
| CV | ＜5.0% |
| Ct | ≤38 |
| LoD | Shandisa makiti kuti uone inoenderana neLoD yemhando yekudzora.a) pasi pe3ng/μL yemusango-yemhando yekumashure, 1% mutation rate inogona kuwonekwa mukuita buffer stably;b) pasi pe1% mutation rate, shanduko ye1 × 103Makopi/mL mune yemusango-mhando kumashure kwe1 × 105Makopi/mL anogona kunyatsowonekwa mukuita buffer;c) iyo IC Reaction Buffer inogona kuona yakaderera yekuona muganho wehutongi SW3 yekambani yemukati kutonga. |
| Zvishandiso Zvinoshanda: | Applied Biosystems 7500 Chaiyo-Nguva PCR SystemsApplied Biosystems 7300 Real-Time PCR Systems, QuantStudio® 5 Chaiyo-Nguva PCR Systems LightCycler®480 Chaiyo-Nguva PCR system BioRad CFX96 Chaiyo-Nguva PCR System |
Kuyerera Kwebasa
Zvinokurudzirwa zvekubvisa reagents: QIAGEN's QIAamp DNA FFPE Tissue Kit (56404), Paraffin-embedded Tissue DNA Rapid Extraction Kit (DP330) yakagadzirwa neTiangen Biotech(Beijing) Co., Ltd.
