Human EGFR Gene 29 Mutations
Product name
HWTS-TM001A-EGFR Yemunhu Gene 29 Mutations Detection Kit (Fluorescence PCR)
Epidemiology
Chirwere chegomarara remapapu chave chiri kukonzera kufa kwegomarara pasi rose, zvichityisidzira hutano hwevanhu zvakanyanya.Isiri-diki kenza yemapapu yemapapu inosvika makumi masere muzana muzana evarwere vekenza yemapapu.EGFR parizvino ndiyo inonyanya kukosha mamorekuru ekurapwa kweasiri diki kenza yemapapu.Iyo phosphorylation yeEGFR inogona kukurudzira tumor cell kukura, kusiyanisa, kupinda, metastasis, anti-apoptosis, uye kukurudzira bundu angiogenesis.EGFR tyrosine kinase inhibitors (TKI) inogona kuvharidzira nzira yeEGFR yekusaina nekudzivisa EGFR autophosphorylation, nekudaro ichidzivirira kuwanda uye kusiyanisa kwema tumor cell, kukurudzira tumor cell apoptosis, kuderedza bundu angiogenesis, nezvimwewo, kuitira kuti uwane bundu rakanangana nekurapa.Nhamba huru yezvidzidzo zvakaratidza kuti kurapa kwakanaka kweEGFR-TKI kwakabatana zvakanyanya nechimiro cheEGFR gene mutation, uye inogona kurambidza zvakananga kukura kwe tumor cell neEGFR gene mutation.Jeni reEGFR riri paruoko rupfupi rwechromosome 7 (7p12), ine hurefu hwakazara hwe200Kb uye ine makumi maviri nemasere exons.Dunhu rakashandurwa rinonyanya kuwanikwa mumaexons 18 kusvika 21, macodons 746 kusvika 753 deletion mutation pa exon 19 accounts angangoita 45% uye L858R mutation pa exon 21 accounts inosvika 40% kusvika 45%.Iyo NCCN Nhungamiro yeKuongororwa uye Kurapwa kweIsiri-Idiki Cell Lung Cancer inotaura zvakajeka kuti EGFR gene mutation test inodiwa pamberi peEGFR-TKI kutonga.Iyi kit yekuyedza inoshandiswa kutungamira kutungamira kwe epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) mishonga, uye inopa hwaro hwemishonga yemunhu kune varwere vane isiri-diki cell kenza yemapapu.Iyi kit inongoshandiswa pakuona kwekuchinja kwakajairika muEGFR gene kune varwere vane isiri-diki cell kenza yemapapu.Mhedzisiro yebvunzo ndeyekuongororwa kwekiriniki chete uye haifanirwe kushandiswa seyo chete hwaro hwekurapwa kwega kwevarwere.Makiriniki anofanirwa kufunga nezvemamiriro emurwere, zviratidzo zvezvinodhaka, uye marapirwo Mabatiro anoita uye zvimwe zviratidzo zvekuongororwa kwerabhoritari uye zvimwe zvinhu zvinoshandiswa kutonga zvizere mhedzisiro yebvunzo.
Channel
| FAM | IC Reaction Buffer, L858R Reaction Buffer, 19del Reaction Buffer, T790M Reaction Buffer, G719X Reaction Buffer, 3Ins20 Reaction Buffer, L861Q Reaction Buffer, S768I Reaction Buffer. |
Technical Parameters
| Storage | Mvura: ≤-18℃ Murima;Lyophilized: ≤30 ℃ Murima |
| Sherufu-hupenyu | Mvura: mwedzi 9;Lyophilized: 12 mwedzi |
| Type Type | nyowani bundu tishu, chando pathological chikamu, parafini-yakamisikidzwa pathological tishu kana chikamu, plasma kana serum. |
| CV | ＜5.0% |
| LoD | nucleic acid reaction solution yekuona pasi pemashure e3ng/μL yemusango-mhando, inogona kuona zvakadzika 1% mutation rate. |
| Zvakananga | Iko hakuna kuyambuka-reactivity ine mhuka yemusango yemunhu genomic DNA uye mamwe marudzi anochinja |
| Zvishandiso Zvishandiso | Applied Biosystems 7500 Chaiyo-Nguva PCR SystemsApplied Biosystems 7300 Chaiyo-Nguva PCR Systems QuantStudio® 5 Chaiyo-Nguva PCR Systems LightCycler® 480 Chaiyo-Nguva PCR system BioRad CFX96 Chaiyo-Nguva PCR System |
Kuyerera Kwebasa
