Human Papillomavirus (Marudzi makumi maviri nemasere) Genotyping Detection Kit (Fluorescence PCR)
Product Name
HWTS-CC004A-Human Papillomavirus (Marudzi makumi maviri nemasere) Genotyping Detection Kit (Fluorescence PCR)
Epidemiology
Iyo kit inoshandisa akawanda nucleic acid amplification (PCR) fluorescence yekuongorora nzira.Yakanyanya kutarisisa maprimers uye probes akagadzirwa zvichibva paL1 gene chinangwa chekutevedzana kweHPV.Iyo chaiyo probe yakanyorwa neFAM fluorophore (HPV6, 16, 26, 40, 53, 58, 73), VIC/HEX fluorophore (HPV11, 18, 33, 43, 51, 59, 81), CY5 fluorophore, HP435 , 45, 54, 56, 68, 82) uye ROX fluorophore (HPV31, 39, 42, 52, 61, 66, 83) pa5', uye boka re3' quencher iBHQ1 kana BHQ2.Munguva yekukwidziridzwa kwePCR, maprimers chaiwo uye maprobes anosunga kune avo akateedzana anoteedzana.Kana Taq enzyme ikasangana nemaprobes akasungirirwa kune yakatarwa kutevedzana, inoita basa re5 'yekupedzisira exonuclease kuparadzanisa mutori wenhau fluorophore kubva kune quencher fluorophore, kuitira kuti iyo fluorescence monitoring system igamuchire chiratidzo chefluorescent, ndiko kuti, pese pese paDNA. strand inokwidziridzwa, fluorescent molecule inoumbwa, iyo inoona kuwiriranisa kwakazara kwekuunganidzwa kweiyo fluorescent masaini uye kuumbwa kwePCR zvigadzirwa, kuitira kuti uwane hunhu uye genotyping kuwanikwa kweiyo nucleic acids e28 marudzi epapillomavirus yemunhu mucervical exfoliated cell samples. .
Channel
| FAM | 16,58,53,73,6,26,40· |
| VIC/HEX | 18,33,51,59,11,81,43 |
| ROX | 31,66,52,39,83,61,42 |
| CY5 | 56,35,45,68,54,44,82 |
Technical Parameters
| Storage | ≤-18℃ Murima |
| Sherufu-hupenyu | 12 mwedzi |
| Type Type | Cervical exfoliated masero |
| Ct | ≤25 |
| CV | ≤5.0% |
| LoD | 25 makopi / maitiro |
| Zvishandiso Zvishandiso | Easy Amp Chaiyo-nguva Fluorescence Isothermal Detection System (HWTS1600)
Applied Biosystems 7500 Chaiyo-Nguva PCR Systems
Applied Biosystems 7500 Fast Real-Time PCR Systems
QuantStudio®5 Chaiyo-Nguva PCR Systems
SLAN-96P Chaiyo-Nguva PCR Systems
LightCycler®480 Chaiyo-Nguva PCR system
LineGene 9600 Plus Chaiyo-Nguva PCR Detection System
MA-6000 Chaiyo-Nguva Quantitative Thermal Cycler
BioRad CFX96 Chaiyo-Nguva PCR System
BioRad CFX Opus 96 Chaiyo-Nguva PCR System |
Kuyerera Kwebasa
Sarudzo 1.
Inokurudzirwa kuburitsa reagent: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).
Sarudzo 2.
Recommended extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) uye Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
