Mycobacterium Tuberculosis Nucleic Acid uye Rifampicin Resistance

Tsanangudzo Pfupi:

Kiti iyi inokodzera kuongororwa kwemhando yeMycobacterium tuberculosis DNA mumasampuli esputum emunhu in vitro, pamwe nekuchinja kwehomozygous munharaunda ye507-533 amino acid codon yerpoB gene iyo inokonzeresa Mycobacterium tuberculosis rifampicin kuramba.

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Product Detail

Product Tags

Product name

HWTS-RT074B-Mycobacterium Tuberculosis Nucleic Acid uye Rifampicin Resistance Detection Kit (Melting Curve)

Chitupa

Epidemiology

Mycobacterium tuberculosis, munguva pfupi seTubercle bacillus, TB, utachiona hwepathogenic hunokonzera tibhii.Parizvino, mishonga inowanzoshandiswa yemutsara wekutanga inorwisa tuberculosis inosanganisira isoniazid, rifampicin nehexambutol, zvichingodaro. Mishonga yepiriviri inorwisa tuberculosis inosanganisira fluoroquinolones, amikacin uye kanamycin, nezvimwewo. Mishonga mitsva yakagadzirwa inonzi linezolid, bedaquiline nedelamani, nezvimwewo. .Zvisinei, nekuda kwekusashandiswa zvisirizvo kwemishonga inorwisa tibhii uye hunhu hwesero rusvingo rwemycobacterium tuberculosis, mycobacterium tuberculosis inokura kurwisa zvinodhaka kumishonga inorwisa tibhii, izvo zvinounza matambudziko akakomba mukudzivirira uye kurapwa kwetibhii.

Rifampicin yakashandiswa zvakanyanya mukurapa varwere vepulmonary tuberculosis kubva mukupera kwema1970, uye ine simba rakakura.Yakave sarudzo yekutanga kupfupisa chemotherapy yevarwere vepulmonary tuberculosis.Kuramba kweRifampicin kunonyanya kukonzerwa nekushandurwa kwejene rerpoB.Kunyange zvazvo mishonga mitsva yekurwisa tibhii iri kuramba ichibuda, uye kushanda kwekiriniki kwevarwere vepulmonary tuberculosis kwakaramba kuchiwedzera, kuchine kushaikwa kwemishonga inorwisa tibhii, uye chiitiko chekushandisa zvinodhaka zvisina musoro mumakiriniki zvakanyanyisa.Zviri pachena kuti Mycobacterium tuberculosis muvarwere vane pulmonary tuberculosis haigoni kuurayiwa zvachose panguva yakakodzera, iyo inoguma inotungamirira kumadhigirii akasiyana-siyana ekudzivirira zvinodhaka mumuviri wemurwere, inowedzera nguva yechirwere, uye inowedzera ngozi yekufa kwemurwere.

Channel

Channel	Zviteshi uye Fluorophores	Reaction Buffer A	Reaction Buffer B	Reaction Buffer C
FAM Channel	Mutori wenhau: FAM, Quencher: Hapana	rpoB 507-514	rpoB 513-520	38KD uye IS6110
CY5 Channel	Mutori wenhau: CY5, Quencher: Hapana	rpoB 520-527	rpoB 527-533	/
HEX (VIC) Channel	Mutori wenhau: HEX (VIC), Quencher: Hapana	Kudzora mukati	Kudzora mukati	Kudzora mukati

Technical Parameters

Storage	≤-18℃ Murima
Sherufu-hupenyu	12 mwedzi
Type Type	Sputum
CV	≤5.0%
LoD	mycobacterium tuberculosis 50 mabhakitiriya / mL rifampicin-resistant mhando yemusango: 2x10³utachiona/mL homozygous mutant: 2x10³utachiona/mL
Zvakananga	Inoona mhuka yemusango mycobacterium tuberculosis uye nzvimbo dzekuchinja kwemamwe majini ekuramba zvinodhaka akadai se katG 315G>C\A, InhA-15C> T, mibairo yebvunzo inoratidza kusaramba rifampicin, zvinoreva kuti hapana kuyambuka-reactivity.
Zvishandiso Zvinoshanda:	SLAN-96P Chaiyo-Nguva PCR Systems BioRad CFX96 Chaiyo-Nguva PCR System LightCycler480® Chaiyo-Nguva PCR System