Staphylococcus Aureus uye Methicillin-Resistant Staphylococcus Aureus
Product name
HWTS-OT062 Staphylococcus Aureus uye Methicillin-Resistant Staphylococcus Aureus Nucleic Acid Detection Kit(Fluorescence PCR)
Chitupa
CE
Epidemiology
Staphylococcus aureus imwe yeakakosha pathogenic mabhakitiriya ehutachiona hweNosocomial.Staphylococcus aureus (SA) ndeye staphylococcus uye inomiririra Gram-positive bacteria, iyo inogona kubereka zvakasiyana-siyana zvehupfu uye invasive enzymes.Iwo mabhakitiriya ane hunhu hwekugovera kwakakura, hwakasimba pathogenicity uye yakakwirira yekupikisa mwero.Thermostable nuclease gene (nuc) ijeni rakachengetedzwa zvakanyanya re staphylococcus aureus.
Channel
| FAM | Methicillin-resistant mecA gene |
| ROX | Kudzora Kwemukati |
| CY5 | staphylococcus aureus nuc gene |
Technical Parameters
| Storage | ≤-18 ℃ & yakachengetedzwa kubva kuchiedza |
| Sherufu-hupenyu | 12 mwedzi |
| Type Type | sputum, ganda uye zvinyoro zvinyoro zvehutachiona hwehutachiona, uye masampuri emumhino |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL methicillin-resistant mabhakitiriya.Kana iyo kiti yaona iyo yenyika LoD referensi, 1000/mL staphylococcus aureus inogona kuonekwa. |
| Zvakananga | The cross-reactivity test inoratidza kuti kit iyi haina muchinjikwa reactivity nemamwe utachiona hwekufema hwakadai se methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, escheritomanniecoccus, ascherichinicoccus, aeruginosa, pneumoniaecoccus, aeruginosa, coagulase-negative staphylococcus. teus mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Zvishandiso Zvishandiso | Applied Biosystems 7500 Chaiyo-Nguva PCR Systems Vadivelu Comedy QuantStudio®5 Chaiyo-Nguva PCR Systems SLAN-96P Chaiyo-Nguva PCR Systems LightCycler®480 Chaiyo-Nguva PCR system LineGene 9600 Plus Chaiyo-Nguva PCR Detection System MA-6000 Chaiyo-Nguva Quantitative Thermal Cycler BioRad CFX96 Chaiyo-Nguva PCR System BioRad CFX Opus 96 Chaiyo-Nguva PCR System |
Kuyerera Kwebasa
Sarudzo 1.
Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) naJiangsu Macro & Micro-Test Med-Tech Co., Ltd. inogona kushandiswa neMacro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS- 3006B).Wedzera 200µL yesaline yakajairwa kumvura yakagadziriswa, uye nhanho dzinotevera dzinofanirwa kutorwa zvinoenderana nemirairo, uye yakakurudzirwa elution vhoriyamu i80µL.
Sarudzo 2.
Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Wedzera 1mL yesaline yakajairika kune inonaya mushure mekugeza nesaline yakajairika, wozosanganisa zvakanaka.Centrifuge pa13,000r/min kwemaminetsi mashanu, bvisa supernatant (chengeta 10-20µL ye supernatant), uye tevera mirairo yekuzochera kunotevera.
Inokurudzirwa yekubvisa reagent: Nucleic Acid Extraction kana Purification Reagent (YDP302) neTiangen Biotech (Beijing) Co.,Ltd.Kubvisa kunofanirwa kuitwa zvakanyatsoenderana nedanho 2 rebhuku rekuraira.Zvinokurudzirwa kushandisa RNase uye DNase-isina mvura yerution ine huwandu hwe100µL.
