Human Methylated NDRG4/SEPT9/SFRP2/BMP3/SDC2 Gene
Product name
HWTS-OT077-Human Methylated NDRG4/SEPT9/SFRP2/BMP3/SDC2 Gene Detection Kit (Fluorescence PCR)
Chitupa
CE
Epidemiology
Muvakuru, anopfuura 10 8 intestinal epithelial masero anodonha kubva pamadziro emukati mazuva ese, uye anoburitswa netsvina kuburikidza nehombe bowel peristalsis.Nekuda kwebundu masero anowanzo kudonha kubva mudumbu turakiti rekupararira kusingaite, chituru chevarwere vematumbo emukati chine masero akawanda anorwara uye abnormal cell components, inova hwaro hwezvinhu hwekuona stool yakagadzikana.Zvidzidzo zvakawana kuti methylation shanduko yevanokurudzira majini chiitiko chekutanga mu tumorigenesis, uye genetic zvinhu zvakawanikwa kubva kune stool samples ye colorectal cancer varwere vanogona kuratidza kuvepo kwegomarara mumatumbo kare.
NDRG4, inozivikanwawo seSMAP-8 uye BDM1, ndeimwe yenhengo ina dzeNDRG gene mhuri (NDRG1-4), iyo yakaratidzwa kuti inobatanidzwa nekuwedzera kwesero, kusiyanisa, kukura uye kushungurudzika.Izvo zvinosimbiswa kuti NDRG4 methylation inogona kuve biomarker yeasina-invasive yekuona kenza colorectal mumasampuli ezvituru.
SEPT9 inhengo yeSeptin gene mhuri, iyo ine angangoita gumi nematatu majini anokodha yakachengetedzwa GTPase domain inogona kusunga mapuroteni ane hukama necytoskeleton, uye inosanganiswa nesero division uye tumorigenesis.Zvidzidzo zvakawana kuti iyo methylated Septin9 gene yemukati inowedzerwa mune stool samples kubva kuvarwere vane colorectal cancer.
Mapuroteni akavanzika ane hukama-ane frizzled (sFRPs) mapuroteni anonyungudika ari kirasi yeWnt nzira vanopikisa nekuda kwehupamhi hwavo hwemaitiro homology kune frizzled (Fz) receptor yeWnt kusaina.Kusashanda kweiyo SFRP gene kunoguma nekusadzora activation yeWnt kusaina kwakabatana ne colorectal cancer.Parizvino, SFRP2 methylation muchituru inogona kushandiswa senge isiri-invasive biomarker yekuongororwa kwe colorectal cancer.
BMP3 inhengo yeTGF-B superfamily uye nekudaro inoita basa rakakosha mukukura kwe embryo nekufurira nekuumba maumbirwo emapfupa ekutanga.BMP3 is hypermethylated mu colorectal cancer uye inogona kushandiswa seyakakosha bundu marker.
SDC2 is cell surface heparan sulfate proteoglycan inobatanidzwa mukugadzirisa kwemaitiro akawanda ehupenyu uye pathological.Kugadziriswa kwemuviri kunosanganisira kuwedzera kwesero, kusiyanisa, kunamatira, sangano re cytoskeletal, kutama, kuporesa maronda, kutaurirana kwesero-matrix, angiogenesis;Pathological maitiro anosanganisira kuzvimba uye cancer.Iyo methylation level yeSDC2 gene mune colorectal cancer tishu yaive yakakwira zvakanyanya kupfuura iyo mune yakajairika tishu.
Channel
| Reaction buffer A | VIC/HEX | methylated NDRG4 gene |
| ROX | methylated SEPT9 gene | |
| CY5 | kutonga kwemukati | |
| Reaction buffer B | VIC/HEX | methylated SFRP2 gene |
| ROX | methylated BMP3 gene | |
| FAM | methylated SDC2 gene | |
| CY5 | kutonga kwemukati |
Dudziro
| Gene | Signal Channel | Ct Kukosha | Dudziro |
| NDRG4 | VIC (HEX) | Ct kukosha≤38 | NDRG4 yakanaka |
| Ct kukosha> 38 kana unde | NDRG4 negative | ||
| SEPT9 | ROX | Ct kukosha≤38 | SEPT9 yakanaka |
| Ct kukosha> 38 kana unde | SEPT9 yakaipa | ||
| SFRP2 | VIC (HEX) | Ct kukosha≤38 | SFRP2 yakanaka |
| Ct kukosha> 38 kana unde | SFRP2 yakaipa |
Technical Parameters
| Storage | Mvura: ≤-18℃ |
| Sherufu-hupenyu | 9 mwedzi |
| Type Type | Stool Sample |
| CV | ≤5.0% |
| Zvakananga | Iko hakuna kuyambuka-reactivity nekenza yechiropa, bile duct cancer, kenza yethyroid uye kenza yemapapu |
| Zvishandiso Zvishandiso | QuantStudio ®5 Chaiyo-Nguva PCR Systems SLAN-96P Chaiyo-Nguva PCR Systems LightCycler®480 Chaiyo-Nguva PCR system LineGene 9600 Plus Chaiyo-Nguva PCR Detection System MA-6000 Chaiyo-Nguva Quantitative Thermal Cycler |
Kuyerera Kwebasa
Inokurudzirwa kubvisa reagent: Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) uye Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS- 3006).
