Human Methylated NDRG4/SEPT9/SFRP2/BMP3/SDC2 Gene

Tsanangudzo Pfupi:

Iyo kiti inotarisirwa kuti in vitro qualitative yekuona yemethylated NDRG4/SEPT9/SFRP2/BMP3/SDC2 majini mumatumbo exfoliated maseru mumasampuli ezvituru zvevanhu.


Product Detail

Product Tags

Product name

HWTS-OT077-Human Methylated NDRG4/SEPT9/SFRP2/BMP3/SDC2 Gene Detection Kit (Fluorescence PCR)

Chitupa

CE

Epidemiology

Muvakuru, anopfuura 10 8 intestinal epithelial masero anodonha kubva pamadziro emukati mazuva ese, uye anoburitswa netsvina kuburikidza nehombe bowel peristalsis.Nekuda kwebundu masero anowanzo kudonha kubva mudumbu turakiti rekupararira kusingaite, chituru chevarwere vematumbo emukati chine masero akawanda anorwara uye abnormal cell components, inova hwaro hwezvinhu hwekuona stool yakagadzikana.Zvidzidzo zvakawana kuti methylation shanduko yevanokurudzira majini chiitiko chekutanga mu tumorigenesis, uye genetic zvinhu zvakawanikwa kubva kune stool samples ye colorectal cancer varwere vanogona kuratidza kuvepo kwegomarara mumatumbo kare.

NDRG4, inozivikanwawo seSMAP-8 uye BDM1, ndeimwe yenhengo ina dzeNDRG gene mhuri (NDRG1-4), iyo yakaratidzwa kuti inobatanidzwa nekuwedzera kwesero, kusiyanisa, kukura uye kushungurudzika.Izvo zvinosimbiswa kuti NDRG4 methylation inogona kuve biomarker yeasina-invasive yekuona kenza colorectal mumasampuli ezvituru.

SEPT9 inhengo yeSeptin gene mhuri, iyo ine angangoita gumi nematatu majini anokodha yakachengetedzwa GTPase domain inogona kusunga mapuroteni ane hukama necytoskeleton, uye inosanganiswa nesero division uye tumorigenesis.Zvidzidzo zvakawana kuti iyo methylated Septin9 gene yemukati inowedzerwa mune stool samples kubva kuvarwere vane colorectal cancer.

Mapuroteni akavanzika ane hukama-ane frizzled (sFRPs) mapuroteni anonyungudika ari kirasi yeWnt nzira vanopikisa nekuda kwehupamhi hwavo hwemaitiro homology kune frizzled (Fz) receptor yeWnt kusaina.Kusashanda kweiyo SFRP gene kunoguma nekusadzora activation yeWnt kusaina kwakabatana ne colorectal cancer.Parizvino, SFRP2 methylation muchituru inogona kushandiswa senge isiri-invasive biomarker yekuongororwa kwe colorectal cancer.

BMP3 inhengo yeTGF-B superfamily uye nekudaro inoita basa rakakosha mukukura kwe embryo nekufurira nekuumba maumbirwo emapfupa ekutanga.BMP3 is hypermethylated mu colorectal cancer uye inogona kushandiswa seyakakosha bundu marker.

SDC2 is cell surface heparan sulfate proteoglycan inobatanidzwa mukugadzirisa kwemaitiro akawanda ehupenyu uye pathological.Kugadziriswa kwemuviri kunosanganisira kuwedzera kwesero, kusiyanisa, kunamatira, sangano re cytoskeletal, kutama, kuporesa maronda, kutaurirana kwesero-matrix, angiogenesis;Pathological maitiro anosanganisira kuzvimba uye cancer.Iyo methylation level yeSDC2 gene mune colorectal cancer tishu yaive yakakwira zvakanyanya kupfuura iyo mune yakajairika tishu.

Channel

Reaction buffer A

VIC/HEX methylated NDRG4 gene
ROX methylated SEPT9 gene
CY5 kutonga kwemukati

Reaction buffer B

VIC/HEX methylated SFRP2 gene
ROX methylated BMP3 gene
FAM methylated SDC2 gene
CY5 kutonga kwemukati

Dudziro

Gene

Signal Channel

Ct Kukosha

Dudziro

NDRG4

VIC (HEX)

Ct kukosha≤38

NDRG4 yakanaka

Ct kukosha> 38 kana unde

NDRG4 negative

SEPT9

ROX

Ct kukosha≤38

SEPT9 yakanaka

Ct kukosha> 38 kana unde

SEPT9 yakaipa

SFRP2

VIC (HEX)

Ct kukosha≤38

SFRP2 yakanaka

Ct kukosha> 38 kana unde

SFRP2 yakaipa

Technical Parameters

Storage Mvura: ≤-18℃
Sherufu-hupenyu 9 mwedzi
Type Type Stool Sample
CV ≤5.0%
Zvakananga Iko hakuna kuyambuka-reactivity nekenza yechiropa, bile duct cancer, kenza yethyroid uye kenza yemapapu
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Kuyerera Kwebasa

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