Munhu ROS1 Fusion Gene Mutation

Tsanangudzo Pfupi:

Iyi kit inoshandiswa ku in vitro qualitative yekuona yemhando gumi neina dzeROS1 fusion gene mutations mumunhu asiri diki cell cancer cancer samples (Tafura 1).Mhedzisiro yebvunzo ndeyekuongororwa kwekiriniki chete uye haifanirwe kushandiswa seyo chete hwaro hwekurapwa kwega kwevarwere.


Product Detail

Product Tags

Product name

HWTS-TM009-Human ROS1 Fusion Gene Mutation Detection Kit (Fluorescence PCR)

Chitupa

CE

Epidemiology

ROS1 inonzi transmembrane tyrosine kinase yemhuri ye insulin receptor.ROS1 fusion gene yakasimbiswa seimwe yakakosha isiri-diki cell cancer yemutyairi gene.Semumiriri weimwe yakasarudzika molecular subtype, chiitiko cheROS1 fusion gene muNSCLC Inenge 1% kusvika 2% ROS1 inonyanya kusangana nekugadzirisa magene mumaexons ayo 32, 34, 35 uye 36. Mushure mekunge yasanganiswa nemajini akadai seCD74, EZR, SLC34A2, uye SDC4, icharamba ichimisa ROS1 tyrosine kinase dunhu.Abnormally activated ROS1 kinase inogona kushandura nzira dzekudzika dzepasi dzakaita seRAS/MAPK/ERK, PI3K/Akt/mTOR, uye JAK3/STAT3, nekudaro vachitora chikamu mukuwanda, kusiyanisa uye metastasis yebundu maseru, uye kukonzera cancer.Pakati peROS1 fusion mutations, CD74-ROS1 inosvika 42%, EZR inosvika 15%, SLC34A2 inoita 12%, uye SDC4 inosvika 7%.Zvidzidzo zvakaratidza kuti iyo ATP-inosunga saiti ye catalytic domain yeROS1 kinase uye iyo ATP-inosunga saiti yeALK kinase ine homology inosvika 77%, saka iyo ALK tyrosine kinase diki molecule inhibitor crizotinib zvichingodaro zvine kurapa kuri pachena. mukurapa kweNSCLC nefusion mutation yeROS1.Nokudaro, kuonekwa kweROS1 fusion mutations ndiyo nheyo uye nheyo yekutungamirira kushandiswa kwemishonga yecrizotinib.

Channel

FAM Reaction Buffer 1, 2, 3 uye 4
VIC(HEX) Reaction Buffer 4

Technical Parameters

Storage

≤-18℃

Sherufu-hupenyu

9 mwedzi

Type Type

parafini-yakaiswa pathological tissue kana sliced ​​samples

CV

<5.0%

Ct

≤38

LoD

Iyi kit inogona kuona fusion shanduko dzakaderera se20 makopi.

Zvishandiso Zvinoshanda:

Applied Biosystems 7500 Chaiyo-Nguva PCR SystemsApplied Biosystems 7500 Fast Real-Time PCR Systems

SLAN ®-96P Chaiyo-Nguva PCR Systems

QuantStudio™ 5 Chaiyo-Nguva PCR Systems

LightCycler®480 Chaiyo-Nguva PCR system

LineGene 9600 Plus Chaiyo-Nguva PCR Detection System

MA-6000 Chaiyo-Nguva Quantitative Thermal Cycler

BioRad CFX96 Chaiyo-Nguva PCR System

BioRad CFX Opus 96 Chaiyo-Nguva PCR System

Kuyerera Kwebasa

Inokurudzirwa yekubvisa reagent: RNeasy FFPE Kit (73504) kubva kuQIAGEN, Paraffin Embedded Tissue Section Total RNA Extraction Kit(DP439) kubva kuTiangen Biotech(Beijing) Co.,Ltd.


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